Mouse monoclonal [KLC264] antibody to Kappa Light Chain (CF® 488).
Specificity
This antibody is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
Applications
Flow Cytometry, IF
Dilutions
Flow Cytometry: 5 µl / million cells or 5 µl / 100 µl whole blood, IF: 1:50-1:100
Reactivity
Human
Immunogen
Recombinant human Ig kappa chain.
Host
Mouse
Clonality
Monoclonal
Clone ID
KLC264
Isotype
IgG1
Light Chains
kappa
Conjugate
CF® 488
Excitation: 490nm, Emission: 515nm
Purification
Protein A/G chromatography.
Concentration
100 µg/ml
Molecular Weight
~22.5 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Store at 4-8°C. Antibody is stable for 24 months.
Synonyms
Ig kappa chain C region, Ig kappa chain C region AG, Ig kappa chain C region CUM, Ig kappa chain C region EU, Ig kappa chain C region OU, Ig kappa chain V-I region HK146, Ig kappa chain V-I region HK189, IGKC, IGKV1D-16, Immunoglobulin kappa constant, Immunoglobulin kappa variable 1D-16
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
