Rabbit polyclonal antibody to JAK1 (phospho Tyr1022 + Tyr1023).
Applicazioni
WB, ICC/IF
Diluizioni consigliate
WB: 1:500-1:1,000, ICC/IF: 1:50-1:200
Reattività
Human, Mouse
Immunogeno
A synthetic phosphorylated peptide around Y1022 & Y1023 of human JAK1 (NP_002218.2).
Sequenza
KEYYTV
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare
Unconjugated
Purificazione
Affinity purification.
Peso molecolare
130 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
JAK 1, JAK 1A, JAK 1B, JAK-1, JAK1A, JAK1B, JAK1_HUMAN, Janus kinase 1, Janus kinase 1 (a protein tyrosine kinase), JTK3, Tyrosine protein kinase JAK 1, Tyrosine protein kinase JAK1, Tyrosine-protein kinase JAK1
Figure 1: Western Blot - Anti-JAK1 (phospho Tyr1022 + Tyr1023) Antibody (A16681)
Western blot analysis of extracts of RAW264. 7 cells, using Anti-JAK1 (phospho Tyr1022 + Tyr1023) Antibody (A16681) at 1:1,000 dilution. RAW264. 7 cells were treated by Pervanadate (1 mM) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunofluorescence analysis of NIH/3T3 cells using Anti-JAK1 (phospho Tyr1022 + Tyr1023) Antibody (A16681) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
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