Rabbit polyclonal antibody to JAK1 (phospho Tyr1022).
Specificità
This antibody detects endogenous levels of JAK1 only when phosphorylated at Tyr1022.
Applicazioni
WB, IHC, ELISA
Diluizioni consigliate
IHC: 1:50-1:100, ELISA: 1:20000
Reattività
Human, Mouse, Rat
Immunogeno
Synthetic peptide derived from human JAK1 around the phosphorylation site of Tyr1022 (amino acids 988-1037).
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare
Unconjugated
Purificazione
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Peso molecolare
131kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
JAK 1, JAK 1A, JAK 1B, JAK-1, JAK1A, JAK1B, JAK1_HUMAN, Janus kinase 1, Janus kinase 1 (a protein tyrosine kinase), JTK3, Tyrosine protein kinase JAK 1, Tyrosine protein kinase JAK1, Tyrosine-protein kinase JAK1
Figure 1: Western Blot - Anti-JAK1 (phospho Tyr1022) Antibody (A94191)
Western blot analysis of lysates from A549 cells using Anti-JAK1 (phospho Tyr1022) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-JAK1 (phospho Tyr1022) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Immunohistochemical analysis of paraffin-embedded human colon tissue using Anti-JAK1 (phospho Tyr1022) Antibody at 1:200 (4°C overnight). Negative control was secondary antibody only.
Immunohistochemical analysis of paraffin-embedded human liver tissue using Anti-JAK1 (phospho Tyr1022) Antibody at 1:200 (4°C overnight). Negative control was secondary antibody only.
Immunofluorescence analysis of mouse liver tissue using Anti-JAK1 (phospho Tyr1022) Antibody (red) at 1:200 (4°C overnight). Cy3 labelled secondary antibody was used at 1:300 (RT 50min). Panel A: Target. Panel B: DAPI. Panel C: Merge.
Immunofluorescence analysis of mouse liver tissue using Anti-JAK1 (phospho Tyr1022) Antibody (red) at 1:200 (4°C overnight). Cy3 labelled secondary antibody was used at 1:300 (RT 50min). Panel A: Target. Panel B: DAPI. Panel C: Merge.
![Western Blot - Anti-JAK1 Antibody [ARC0434] (A80915) - Antibodies.com](https://cdn.antibodies.com/image/catalog/80/A80915_1.jpg?profile=product_alternative)
