This antibody recognises human interferon regulatory factor 5 (IRF5), a ~58 kDa member of the IRF family which acts as a key regulator of interferon gene transcription, and is expressed in B cells and dendritic cells, and is inducible by type I interferon (IFN) in most lymphoid cells.The role of IRF3 and IRF7 as transcriptional mediators of virus- and IFN-induced signaling pathways is well documented. IRF5 can also regulate IFN gene transcription, but appears to be more restricted in its cellular expression and range of viral stimuli, and has the ability to induce a wide range of chemokines involved in T-lymphocyte recruitment, including RANTES, MCP-1 and IP-10.

Applicazioni

ELISA, WB, IHC-P, IP

Diluizioni consigliate

IHC-P 1: 1:50, WB: 1:1,000 - 1:2,000

Reattività

Human

Immunogeno

Histidine-tagged recombinant peptide corresponding to amino acids 176-240 of human IRF5, purified from E. coli.

Sequenza

PPTLQPPTLQPPVVLGPPAPDPSPLAPPPGNPAGFRELLSEVLEPGPLPASLPPAGEQLLPDLLI

Specie ospite

Mouse

Clonalità

Monoclonal

Clona ID

10T1

Isotipo

IgG1

Coniugare

Unconjugated

Purificazione

Protein A affinity chromatography of ascites.

Concentrazione

1 mg/ml

Peso molecolare

Approximately 58 kDa in THP-1 cell lysates.

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline with 10% Glycerol and 0.02% Sodium Azide.

Conservazione

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Note generali

Mouse anti Human IRF5 antibody, clone 10T1 recognizes human interferon regulatory factor 5 (IRF5), a ~58 kDa member of the IRF family which acts as a key regulator of interferon gene transcription, and is expressed in B cells and dendritic cells, and is inducible by type I interferon (IFN) in most lymphoid cells.The role of IRF3 and IRF7 as transcriptional mediators of virus- and IFN-induced signaling pathways is well documented. IRF5 can also regulate IFN gene transcription, but appears to be more restricted in its cellular expression and range of viral stimuli, and has the ability to induce a wide range of chemokines involved in T-lymphocyte recruitment, including RANTES, MCP-1 and IP-10 (Barnes et al. 2002).

Sinonimi

Interferon regulatory factor 5, Interferon regulatory factor 5 bone marrow variant, IRF 5, IRF-5, IRF5_HUMAN, SLEB10

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Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.