Anti-IRAK4 Anticorpo (A84059)

Name: Anti-IRAK4 Antibody
Brand: Antibodies.com
SKU: A84059
Price: 480.0 USD
Availability: InStock

Questo prodotto è identico a Abcam (ab113525). Scopri di più con meno.

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$480

Anticorpo policlonale di capra contro IRAK4 per ELISA, WB, IHC, IF e Flow Cytometry.

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Consegna

Spese di spedizione

$40

Tempi di consegna

3-5 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-IRAK4 Antibody

Descrizione

Goat polyclonal antibody to IRAK4.

Applicazioni

ELISA, WB, IHC, IF, Flow Cytometry

Diluizioni consigliate

ELISA: 1:32,000, WB: 0.5-2µg/ml, IHC: 3.75µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reattività

Human

Immunogeno

Synthetic peptide corresponding to the N terminal region of human IRAK4.

Sequenza

NKPITPSTYVRC

Specie ospite

Goat

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentrazione

500 µg/ml

Peso molecolare

50 kDa

MW previsto

51.5kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Sinonimi

IL-1 receptor-associated kinase 4, Interleukin 1 receptor associated kinase 4 mutant form 1, Interleukin-1 receptor-associated kinase 4, Interleukin1 receptor associated kinase 4, IPD1, IRAK 4, IRAK-4, IRAK4 mutated form 1, IRAK4_HUMAN, LOC 51135, NY REN 64, NY REN 64 antigen, NY-REN-64, REN64, Renal carcinoma antigen NY-REN-64

Controlli isotipo

Goat IgG (A121671)

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (5)

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Figure 1: Western Blot - Anti-IRAK4 Antibody (A84059)

IRAK4 expression in HeLa (A) and Jurkat (B) cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-IRAK4 Antibody (A84059) at 1µg/ml and detected by chemiluminescence.

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Figure 2: Immunofluorescence - Anti-IRAK4 Antibody (A84059)

IRAK4 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-IRAK4 Antibody (A84059) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and microtubule staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Flow Cytometry - Anti-IRAK4 Antibody (A84059)

IRAK4 expression in HeLa cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-IRAK4 Antibody (A84059) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

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Figure 4: Immunohistochemistry - Anti-IRAK4 Antibody (A84059)

IRAK4 expression in Human Heart analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-IRAK4 Antibody (A84059) at 3.75µg/ml and revealed with alkaline phosphatase (AP).