Western Blot - Anti-Integrin beta 3 Antibody [ARC0460] (A305528)
Western blot analysis of extracts of various cell lines, using Anti-Integrin beta 3 Antibody [ARC0460] (A305528) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded rat bone marrow using Anti-Integrin beta 3 Antibody [ARC0460] (A305528) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse bone marrow using Anti-Integrin beta 3 Antibody [ARC0460] (A305528) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Western Blot - Anti-Integrin beta 3 Antibody [ARC0460] (A305528)
Immunoprecipitation analysis of 300µg extracts of U-87MG cells using 3µg of Anti-Integrin beta 3 Antibody [ARC0460] (A305528). This Western blot was performed on the immunoprecipitate using Anti-Integrin beta 3 Antibody [ARC0460] (A305528) at a dilution of 1:1000.
Flow cytometry analysis of HEL cells, stained with Rabbit IgG isotype control (2.5 µg/ml, blue line) or Anti-Integrin beta 3 Antibody [ARC0460] (A305528), (2.5 µg/ml orange line), followed by FITC conjugated goat anti-rabbit polyclonal antibody (1:200 dilution) staining. Non-fluorescently stained HEL cells, used as blank control (red line).
Flow cytometry analysis of U937 cells, stained with Rabbit IgG isotype control (2.5 µg/ml, blue line) or Anti-Integrin beta 3 Antibody [ARC0460] (A305528), (2.5 µg/ml orange line), followed by FITC conjugated goat anti-rabbit polyclonal antibody (1:200 dilution). Non-fluorescently stained U937 cells, used as blank control (red line).
![Western Blot - Anti-Integrin beta 3 Antibody [ARC0460] (A305528) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305528_1.jpg?profile=product_top)
![Immunohistochemistry - Anti-Integrin beta 3 Antibody [ARC0460] (A305528) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305528_2.jpg?profile=product_top)
![Immunohistochemistry - Anti-Integrin beta 3 Antibody [ARC0460] (A305528) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305528_3.jpg?profile=product_top)
![Western Blot - Anti-Integrin beta 3 Antibody [ARC0460] (A305528) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305528_4.jpg?profile=product_top)
![Flow Cytometry - Anti-Integrin beta 3 Antibody [ARC0460] (A305528) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305528_5.jpg?profile=product_top)
![Flow Cytometry - Anti-Integrin beta 3 Antibody [ARC0460] (A305528) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305528_6.jpg?profile=product_top)
![Western Blot - Anti-Integrin beta 3 Antibody [ARC0460] (A305528) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305528_1.jpg?profile=product_top_thumb)
![Immunohistochemistry - Anti-Integrin beta 3 Antibody [ARC0460] (A305528) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305528_2.jpg?profile=product_top_thumb)
![Immunohistochemistry - Anti-Integrin beta 3 Antibody [ARC0460] (A305528) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305528_3.jpg?profile=product_top_thumb)
![Western Blot - Anti-Integrin beta 3 Antibody [ARC0460] (A305528) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305528_4.jpg?profile=product_top_thumb)
![Flow Cytometry - Anti-Integrin beta 3 Antibody [ARC0460] (A305528) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305528_5.jpg?profile=product_top_thumb)
![Flow Cytometry - Anti-Integrin beta 3 Antibody [ARC0460] (A305528) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305528_6.jpg?profile=product_top_thumb)
![Western Blot - Anti-Integrin beta 3 Antibody [ARC0460] (A305528) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305528_1.jpg?profile=product_image)
![Immunohistochemistry - Anti-Integrin beta 3 Antibody [ARC0460] (A305528) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305528_2.jpg?profile=product_image)
![Immunohistochemistry - Anti-Integrin beta 3 Antibody [ARC0460] (A305528) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305528_3.jpg?profile=product_image)
![Western Blot - Anti-Integrin beta 3 Antibody [ARC0460] (A305528) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305528_4.jpg?profile=product_image)
![Flow Cytometry - Anti-Integrin beta 3 Antibody [ARC0460] (A305528) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305528_5.jpg?profile=product_image)
![Flow Cytometry - Anti-Integrin beta 3 Antibody [ARC0460] (A305528) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305528_6.jpg?profile=product_image)
![Immunohistochemistry - Anti-Integrin beta 3 Antibody [ITGB3/2145] (A249082) - Antibodies.com](https://cdn.antibodies.com/image/catalog/249/A249082_1.jpg?profile=product_alternative)
![SDS-PAGE - Anti-Integrin beta 3 Antibody [Y2/51] (A249079) - Antibodies.com](https://cdn.antibodies.com/image/catalog/249/A249079_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Integrin beta 3 Antibody [ITGB3/2145] - BSA and Azide free (A252262) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252262_1.jpg?profile=product_alternative)
![SDS-PAGE - Anti-Integrin beta 3 Antibody [Y2/51] - BSA and Azide free (A252259) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252259_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Integrin beta 3 Antibody [RM382] (A121453) - Antibodies.com](https://cdn.antibodies.com/image/catalog/121/A121363_1.png?profile=product_alternative)
![Immunohistochemistry - Anti-Integrin beta 3 Antibody [ITGB3/2166R] - BSA and Azide free (A252265) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252265_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Integrin beta 3 Antibody [ITGB3/2166R] (A249085) - Antibodies.com](https://cdn.antibodies.com/image/catalog/249/A249085_1.jpg?profile=product_alternative)