Contattaci
+1 (314) 370-6046 oContattaci
Visit our English Website
Antibodies.com LogoAntibodies.com Logo
Accedi / RegistratiIl Mio Account
0

Anti-Virus dell'Influenza A Nucleoprotein Anticorpo [C43] (A335)

Questo prodotto è identico a Abcam (ab128193). Scopri di più con meno.

Immunofluorescence assay of MDCK cells derived from canine kidney cells, and A549 cells derived from human lung carcinoma cells, that were infected with H1N1 influenza virus (A/PuertoRico/8/34). Samples were taken at 3, 9, and 24 hours post-infection. C43 antibody efficiently detected virus-infected MDCK and A549 cells as early as 3 h after infection. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further reaction with an Alexa Fluor 488-conjugated secondary antibody Immunofluorescence assay of 293T cells expressing HA or NP of pandemic (H1N1) 2009 influenza A virus (A/Suita/1/2009). C43 specifically recognized NP-expressing cells while a commercially available mouse anti-HA monoclonal antibody specifically recognized HA Western blotting of MDCK cells infected with H1N1 (A/PuertoRico/8/34), H5N1 (A/duck/HK/342/78), or H5N2 (A/crow/Kyoto/53/04) using C43 as a primary antibody. Samples were collected at 3, 9, 24, and 48 hours post-infection. C43 detected NP after 3 hours post-infection and detected three different types of influenza viruses. Identificationof Influenza Nucleoprotein in crude extract of MDCK cells infected with Influenza A virus (H1N1) PuertoRico/8/34 using C43 mnoclonal antibody. 10-20% gradient gel Blotting 15v, 30min (semi-dry) blocking over night, 4? 1st antibody 1/1000 dilution 2nd antibody 1/10,000 dilution; rabbit polyclonal secodary antibody to mouse IgG- H & L (HRP) (ab97046; abcam). Positions of molecular size markers are shown in kDa on the left. NP size is 56 kDa according to Swiss-Prot.
$535

Anticorpo monoclonale [C43] di topo contro Influenza A Virus Nucleoprotein per WB, IP, IF, IHC e ELISA.

Garanzia al 100%
Garanzia di corrispondenza del prezzo

Spese di spedizione
$40
Tempi di consegna
4-6 Giorni lavorativi

Telefono
+1 (314) 370-6046
Lun - Ven, 8:00 - 16:00 AST
E-mail
orders@antibodies.com

Nome
Anti-Influenza A Virus Nucleoprotein Antibody [C43]
Descrizione
Mouse monoclonal (C43) antibody to Influenza A Virus Nucleoprotein.
Specificità
This antibody reacts with NP of all Influenza A viruses tested so far, including seasonal H2N2, H3N2, and avian H5N1, H5N2, and H1N1 (seasonal, pandemic, and swine).
Applicazioni
WB, IP, IF, IHC, ELISA
Diluizioni consigliate
WB: 1:300-1:1,000, IP: 1:100, IF: 1:200, IHC: 1:200
Reattività
Influenza A Virus
Reattività incrociata
This antibody does not react with Influenza B viruses.
Immunogeno
Human Influenza A Virus (H2N2) Okada strain.
Specie ospite
Mouse
Clonalità
Monoclonal
Clona ID
C43
Isotipo
IgG2a
Coniugare

Unconjugated

Purificazione
Affinity purification.
Concentrazione
1 mg/ml
Purezza
> 90% (by SDS-PAGE)
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline with 50% Glycerol (filter sterilised, without Sodium Azide, and carrier free).
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
Common flu NP, Influenza A virus NP, NP, Nucleocapsid protein, Nucleoprotein, Protein N, Seasonal Influenza A (H1N1) Nucleocapsid Protein
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Immunofluorescence assay of MDCK cells derived from canine kidney cells, and A549 cells derived from human lung carcinoma cells, that were infected with H1N1 influenza virus (A/PuertoRico/8/34). Samples were taken at 3, 9, and 24 hours post-infection. C43 antibody efficiently detected virus-infected MDCK and A549 cells as early as 3 h after infection. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further reaction with an Alexa Fluor 488-conjugated secondary antibodyClicca sull'immagine per la visualizzazione estesa
Figure 1: Immunofluorescence - Anti-Influenza A Virus Nucleoprotein Antibody [C43] (65-110)
Anti-Influenza A Virus Nucleoprotein Antibody
Immunofluorescence assay of 293T cells expressing HA or NP of pandemic (H1N1) 2009 influenza A virus (A/Suita/1/2009). C43 specifically recognized NP-expressing cells while a commercially available mouse anti-HA monoclonal antibody specifically recognized HAClicca sull'immagine per la visualizzazione estesa
Figure 2: Immunofluorescence - Anti-Influenza A Virus Nucleoprotein Antibody [C43] (65-110)
Anti-Influenza A Virus Nucleoprotein Antibody
Western blotting of MDCK cells infected with H1N1 (A/PuertoRico/8/34), H5N1 (A/duck/HK/342/78), or H5N2 (A/crow/Kyoto/53/04) using C43 as a primary antibody. Samples were collected at 3, 9, 24, and 48 hours post-infection. C43 detected NP after 3 hours post-infection and detected three different types of influenza viruses.Clicca sull'immagine per la visualizzazione estesa
Figure 3: Anti-Influenza A Virus Nucleoprotein Antibody [C43] (65-110)
Anti-Influenza A Virus Nucleoprotein Antibody
Identificationof Influenza Nucleoprotein in crude extract of MDCK cells infected with Influenza A virus (H1N1) PuertoRico/8/34 using C43 mnoclonal antibody. 10-20% gradient gel Blotting 15v, 30min (semi-dry) blocking over night, 4? 1st antibody 1/1000 dilution 2nd antibody 1/10,000 dilution; rabbit polyclonal secodary antibody to mouse IgG- H & L (HRP) (ab97046; abcam). Positions of molecular size markers are shown in kDa on the left. NP size is 56 kDa according to Swiss-Prot.Clicca sull'immagine per la visualizzazione estesa
Figure 4: Anti-Influenza A Virus Nucleoprotein Antibody [C43] (65-110)
Anti-Influenza A Virus Nucleoprotein Antibody

Prodotti alternativi a Anti-Virus dell'Influenza A Nucleoprotein Anticorpo [C43] (A335)

ELISA - Anti-Influenza A Virus Nucleoprotein Antibody [19C10] (A325385) - Antibodies.com
(2)
A325385
Anti-Virus dell'Influenza A Nucleoprotein Anticorpo [19C10]
Anticorpo monoclonale [19C10] di topo contro Influenza A Virus Nucleoprotein per FociSpot e ELISA.
Segnaposto immagine Antibodies.com - Scopri di più spendendo meno
A280437
Anti-Virus dell'Influenza A Nucleoprotein Anticorpo [F8]
Anticorpo monoclonale [F8] di topo contro Influenza A Virus Nucleoprotein per ELISA e IHC-Fr.
Immunohistochemical analysis - Anti-Influenze A IP Antibody (HB65) - Antibodies.com
A93427
Anti-Virus dell'Influenza A Nucleoprotein Anticorpo [HB65]
Anticorpo monoclonale [HB65] di topo contro Influenza A Virus Nucleoprotein per ELISA, WB, IHC e IHC-P.
ELISA - Anti-Influenza A Virus Nucleoprotein Antibody [19C10] (A325385) - Antibodies.com
(2)
A325385
Anti-Virus dell'Influenza A Nucleoprotein Anticorpo [19C10]
Anticorpo monoclonale [19C10] di topo contro Influenza A Virus Nucleoprotein per FociSpot e ELISA.
Segnaposto immagine Antibodies.com - Scopri di più spendendo meno
A280437
Anti-Virus dell'Influenza A Nucleoprotein Anticorpo [F8]
Anticorpo monoclonale [F8] di topo contro Influenza A Virus Nucleoprotein per ELISA e IHC-Fr.
Immunohistochemical analysis - Anti-Influenze A IP Antibody (HB65) - Antibodies.com
A93427
Anti-Virus dell'Influenza A Nucleoprotein Anticorpo [HB65]
Anticorpo monoclonale [HB65] di topo contro Influenza A Virus Nucleoprotein per ELISA, WB, IHC e IHC-P.
Menù Principale Contattaci 0Check-out
Inizio pagina