Anti-Indoleamine 2, 3-dioxygenase Antibody [ARC0560] (A306280)

Western blot analysis of extracts of Mouse ovary cells, using Anti-Indoleamine 2, 3-dioxygenase Antibody [ARC0560] (A306280) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

Immunohistochemistry analysis of paraffin-embedded human liver using Anti-Indoleamine 2, 3-dioxygenase Antibody [ARC0560] (A306280) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Anti-Indoleamine 2, 3-dioxygenase Antibody [ARC0560] (A306280) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.