Figure 1: Western Blot - Anti-IL2 Receptor beta / p75 Antibody (A9815)
Western blot analysis of extracts of various cell lines, using Anti-IL2 Receptor beta / p75 Antibody (A9815) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Figure 2: Western Blot - Anti-IL2 Receptor beta / p75 Antibody (A9815)
Western blot analysis of extracts of RAW264. 7 cells, using Anti-IL2 Receptor beta / p75 Antibody (A9815) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
