Anti-IL-8 Antibody - BSA and Azide free (A283926)

Anti-IL-8 Antibody - BSA and Azide free

Rabbit polyclonal antibody to IL-8.

This antibody recognises human IL-8 (Interleukin-8), a 72 amino acid pro-inflammatory CXC chemokine designated CXCL8, secreted by endothelial cells and monocytes, which acts as a major chemoattractant for neutrophils and also T cells and basophils.IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-Terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Previous nomenclature for IL-8 includes neutrophil activating protein 1(NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C.

IF, WB, ELISA, IHC-P, Functional Assays

ELISA: 0.5 - 2 µg/ml, Functional Assays: 2.0 - 3.0 µg/ml, IHC-P: 0.25 - 1 µg/ml, WB: 0.1 - 0.2 µg/ml

Human

Recombinant human IL-8.

Rabbit

Polyclonal

IgG

1 mg/ml after reconstitution.

Reconstitute with 0.1 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. For functional studies do not add sodium azide

Supplied in Phosphate Buffered Saline.

Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Rabbit anti Human Interleukin-8 antibody recognizes human IL-8 (Interleukin-8), a 72 amino acid pro-inflammatory CXC chemokine designated CXCL8, secreted by endothelial cells and monocytes, which acts as a major chemoattractant for neutrophils and also T cells and basophils.IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-Terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Previous nomenclature for IL-8 includes neutrophil activating protein 1(NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C.

C-X-C motif chemokine 8, C-X-C motif) ligand 8, Chemokine, CXCL8, Emoctakin, GCP-1, Granulocyte chemotactic protein 1, IL8, Interleukin-8, MDNCF, Monocyte-derived neutrophil chemotactic factor