Western blot analysis of THP-1, using Anti-IL-8 Antibody (A14047) at 1:700 dilution. THP-1 cells were treated by PMA/TPA (80 nM) at 37°C for overnight and LPS (1 µg/ml) at 37°C for 6 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Western blot analysis of Recombinant Human IL-8 Protein, using Anti-IL-8 Antibody (A14047) at 1:700 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
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