Anti-IL-4 Anticorpo [CC313] (A280035)

Anti-IL-4 Antibody [CC313]

Mouse monoclonal [CC313] antibody to IL-4.

This antibody recognises bovine interleukin 4 (IL-4), also known as B-cell stimulatory factor 1. Bovine interleukin-4 is a 15 kDa, 135 amino acid peptide which is processed to a mature secreted form with the cleavage of a 24 amino acid, N-terminal signal peptide resulting in a 12.6 kDa active cytokine, the apparent MW may be altered by glycosylation. IL-4 is expressed by a range of cells including T and B cells, macrophages and monocytes. It plays an important role in the regulation of bovine T and B cell responses. Mouse anti Bovine interleukin-4, clone CC313 has been used successfully as a coating antibody in a sandwich ELISA with biotinylated Mouse anti Bovine IL-4 antibody, clone CC314 (MCA2372B) as a detection reagent both with bovine and ovine samples. Clones CC313 and CC314 have been used in the reverse configuration for the measurement of interleukin-4 in caprine samples.

ELISA

ELISA: 2 µg/ml - 5 µg/ml

Bovine, Sheep, Goat

Recombinant bovine IL-4.

Mouse

Monoclonal

CC313

IgG2a

Unconjugated

Protein A affinity chromatography of tissue culture supernatant.

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Bovine interleukin-4, clone CC313 is specific for bovine interleukin 4 (IL-4), also known as B-cell stimulatory factor 1. Bovine interleukin-4 is a 15 kDa, 135 amino acid peptide which is processed to a mature secreted form with the cleavage of a 24 amino acid, N-terminal signal peptide resulting in a 12.6 kDa active cytokine, the apparent MW may be altered by glycosylation (Heussler et al. 1992).IL-4 is expressed by a range of cells including T and B cells, macrophages and monocytes. It plays an important role in the regulation of bovine T and B cell responses.Mouse anti Bovine interleukin-4, clone CC313 has been used successfully as a coating antibody in a sandwich ELISA with biotinylated Mouse anti Bovine IL-4 antibody, clone CC314 (MCA2372B) as a detection reagent both with bovine (Stabel et al. 2011) and ovine samples (Olivier et al. 2012). Clones CC313 and CC314 have been used in the reverse configuration for the measurement of interleukin-4 in caprine samples (Marinaro et al. 2012).

B cell growth factor 1, B cell IgG differentiation factor, B cell stimulatory factor 1 , B-cell stimulatory factor 1, BCGF 1, BCGF1, Binetrakin , BSF 1, BSF-1, BSF1 , IGG1 induction factor, IL 4, IL4, Il4e12, IL4_HUMAN, Interleukin 4, Interleukin 4 variant 2, Interleukin 4, isoform 1, Interleukin-4, Lymphocyte stimulatory factor 1, MGC79402, Pitrakinra