This antibody recognises natural and recombinant mouse IL-4. Clone BVD4-1D11 is reported to neutralize IL-4 activity.
Applications
WB, Flow Cytometry, IHC-Fr, ELISA
Dilutions
ELISA: 1 µg/ml - 5 µg/ml, FC 1: 1:10 - 1:50, WB: 1 µg/ml - 5 µg/ml, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl
Reactivity
Mouse
Cross Reactivity
This antibody does not cross react with Rat
Immunogen
Recombinant mouse IL-4.
Host
Rat
Clonality
Monoclonal
Clone ID
BVD4-1D11
Isotype
IgG2b
Conjugate
Unconjugated
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rat anti Mouse Interleukin-4 antibody, clone BVD4-1D11 recognizes natural and recombinant mouse IL-4. Clone BVD4-1D11 is reported to neutralize IL-4 activity (Abrams et al., 1992).