Anti-IL-4 Anticorpo (A282941)

Anti-IL-4 Antibody - BSA and Azide free

Rabbit polyclonal antibody to IL-4.

This antibody recognises mouse interleukin-4 (IL-4), also known as B-cell stimulatory factor 1, a 120 amino acid pleiotropic cytokine cleaved from a 140 amino acid precursor which interacts with multiple cell lineages, including T cells, fibroblasts and macrophages. Interleukin-4 is one of the most important factors involved in B cell IgG1 and IgE class switching. IL-4 acts as a regulator of low affinity Fc receptor for IgE (CD23) expression on both lymphocytes and monocytes (Miyajima et al. 1991, Hudak et al. 1987). IL-4 acts through binding to it's cognate receptor CD124, a receptor which also binds the related cytokine IL-13.Interleukin -4 plays an important role in the alternative activation of murine macrophages as indicated by the ability to potently up-regulate the expression and activity of CD206, the macrophage mannose receptor.

WB, ELISA, Functional Assays

ELISA: 0.5 µg/ml, Functional Assays: 0.25 µg/ml - 0.40 µg/ml, WB: 0.1 µg/ml - 0.2 µg/ml

Mouse

Recombinant mouse IL-4.

Rabbit

Polyclonal

IgG

Unconjugated

1 mg/ml after reconstitution.

Reconstitute with 0.1 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended..NB: For functional studies do not add sodium azide.

Supplied in Phosphate Buffered Saline.

Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Rabbit anti Mouse Interleukin-4 antibody recognizes mouse interleukin-4 (IL-4), also known as B-cell stimulatory factor 1, a 120 amino acid pleiotropic cytokine cleaved from a 140 amino acid precursor which interacts with multiple cell lineages, including T cells, fibroblasts and macrophages (Crawford et al. 1987).Interleukin-4 is one of the most important factors involved in B cell IgG1 and IgE class switching (Winter et al. 1987). IL-4 acts as a regulator of low affinity Fc receptor for IgE (CD23) expression on both lymphocytes and monocytes (Miyajima et al. 1991, Hudak et al. 1987). IL-4 acts through binding to it's cognate receptor CD124, a receptor which also binds the related cytokine IL-13.Interleukin -4 plays an important role in the alternative activation of murine macrophages as indicated by the ability to potently up-regulate the expression and activity of CD206, the macrophage mannose receptor (Stein et al. 1992).

B cell growth factor 1, B cell IgG differentiation factor, B cell stimulatory factor 1 , B-cell stimulatory factor 1, BCGF 1, BCGF1, Binetrakin , BSF 1, BSF-1, BSF1 , IGG1 induction factor, IL 4, IL4, Il4e12, IL4_HUMAN, Interleukin 4, Interleukin 4 variant 2, Interleukin 4, isoform 1, Interleukin-4, Lymphocyte stimulatory factor 1, MGC79402, Pitrakinra