IKK beta (phospho Tyr188) Cell Based Kit Elisa (A102574)

Nome
IKK beta (phospho Tyr188) Cell Based ELISA Kit
Tipo di kit
Cell-based (qualitative)
Principio di kit
This IKK beta (phospho Tyr188) Cell Based ELISA Kit allows for the detection of IKK beta (phospho Tyr188) and the effects that certain stimulation conditions have on IKK beta (phospho Tyr188) expression in different cell lines. Qualitative determination of IKK beta (phospho Tyr188) concentration is achieved by an indirect ELISA format. In essence, the IKK beta (phospho Tyr188) is captured by Anti-IKK beta (phospho Tyr188) Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this IKK beta (phospho Tyr188) Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) Anti-IKK beta Antibody is provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.
Piattaforma
Microplate
Tipo di rilevamento
Colorimetric
Tipo di campione
Adherent cells and suspension cells.
Campo di rilevamento
> 5000 Cells
Tempo del kit
4 hours 30 minutes
Reattività
Human, Mouse, Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Conservazione
Store at + 4°C. Stable for 6 months.
Sinonimi
I kappa B kinase 2, I kappa B kinase beta, I-kappa-B kinase 2, I-kappa-B-kinase beta, IkBKB, IKK-B, IKK-beta, IKK2, IKKB, IKKB_HUMAN, IMD15, Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase beta, Inhibitor of nuclear factor kappa-B kinase subunit beta, NFKBIKB, Nuclear factor NF-kappa-B inhibitor kinase beta
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

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