Rabbit polyclonal antibody to IkB-b (Phospho-Ser23)
Specificity
The antibody detects endogenous level of IkB-b only when phosphorylated at serine 23.
Applications
WB, IHC
Dilutions
Immunohistochemistry: 1:50~1:100
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 23 (L-G-S(p)-L-G) derived from Human IkB-b.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Predicted MW
48kd
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Synonyms
I-kappa-B-beta, IkappaBbeta, IkB-beta, IKBB, NF-kappa-B inhibitor beta, NF-kappa-BIB, NFKBIB, Thyroid receptor-interacting protein 9, TR-interacting protein 9, TRIP-9, TRIP9
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB-b (Phospho-Ser23) Antibody #11304 (left) or the same antibody preincubated with blocking peptide (right).
