Rabbit polyclonal antibody to Insulin degrading enzyme/IDE.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:10-1:100
Reactivity
Human, Mouse
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human Insulin-degrading enzyme (Insulin-degrading enzyme (IDE)) (NP_004960.2).
Western Blot - Anti-Insulin degrading enzyme/IDE Antibody (A13561)
Western blot analysis of extracts of various cell lines, using Anti-Insulin degrading enzyme/IDE Antibody (A13561) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit.
Western Blot - Anti-Insulin degrading enzyme/IDE Antibody (A13561)
Western blot analysis of extracts from normal (control) and Insulin-degrading enzyme (Insulin-degrading enzyme (IDE)) knockout (KO) HeLa cells, using Anti-Insulin degrading enzyme/IDE Antibody (A13561) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
