Human VEGF Receptor 2 ELISA Kit (A1537)

Human VEGF Receptor 2 ELISA Kit

Sandwich (quantitative)

Human VEGF R2 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human VEGF R2 in the samples. A monoclonal antibody specific for human VEGF R2 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for VEGF R2, and VEGF R2 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of VEGF R2 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

Cell culture supernatant, serum and plasma.

62.50 - 4000 pg/ml

This kit can assay both natural and recombinant human VEGF R2.

Human

This assay has shown no significant cross-reactivity.

94% (Average Recovery from Serum Samples).

Store at 2 - 8°C.

CD309, Fetal liver kinase 1, FLK-1, FLK1, KDR, Kinase insert domain receptor, Protein-tyrosine kinase receptor flk-1, Vascular endothelial growth factor receptor 2, VEGFR-2, VEGFR2