Umano TNF beta Kit Elisa (A721)

Human TNF beta ELISA Kit

Sandwich (quantitative)

This TNF beta enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-TNF beta Antibody. Standards or samples are then added to the microtire plate wells and TNF beta, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of TNF beta present in the sample, Anti-TNF beta Antibody (Biotin) is added to each well to "sandwich" the TNF beta immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TNF beta will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Microplate

Serum, plasma, body fluids, tissue lysates, and cell culture supernatants.

< 5 pg/ml

62.5-4000 pg/ml

Human

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Store at 2-8°C for 4 months or at -20°C for 8 months.

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.