Human TIM-1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human TIM-1 in the samples. A monoclonal antibody specific for human TIM-1 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for TIM-1, and TIM-1 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of TIM-1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Piattaforma

Microplate

Tipo di rilevamento

Colorimetric

Tipo di campione

Cell culture supernatant, serum and plasma.

Sensibilità

1.42 pg/ml

Campo di rilevamento

31.25 - 2000 pg/ml

Recupero

102% (Average Recovery from Serum Samples).

Specificità

This kit can assay both natural and recombinant human TIM-1.

Reattività

Human

Reattività incrociata

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Conservazione

Store at 2 - 8°C.

Sinonimi

CD365, HAVCR, HAVCR 1, HAVcr-1, Havcr1, Hepatitis A virus cellular receptor 1, Kidney injury molecule 1, KIM 1, KIM-1, T cell immunoglobin domain and mucin domain protein 1, T cell immunoglobulin mucin family member 1, T cell immunoglobulin mucin receptor 1, T-cell immunoglobulin and mucin domain-containing protein 1, T-cell membrane protein 1, TIM, TIM 1, TIM1, TIMD 1, TIMD-1, TIMD1, TIMD1_HUMAN

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.