Umano Thrombospondin-1 Kit Elisa (A1524)

Human Thrombospondin-1 ELISA Kit

Sandwich (quantitative)

Human Thrombospondin-1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human THBS-1 in the samples. A monoclonal antibody specific for human THBS-1 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for THBS-1, and THBS-1 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of THBS-1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

26.39 pg/ml

0.78 - 50 ng/ml

This kit can assay both natural and recombinant human THBS-1.

Human

This assay has shown no significant cross-reactivity.

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Store at 2 - 8°C.

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.