Human Substance P ELISA Kit is a competitive (quantitative) Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of human Substance P in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Tipo di kit

Competitive (quantitative)

Principio di kit

Human Substance P ELISA Kit employs the Competitive Enzyme Immunoassay (ELISA) technique for the quantitative measurement ofhumanSubstance P in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. A monoclonal antibody specific for Substance P has been pre-coated onto a 96-well microtiter plate. During the incubation, Substance P in the sample or standard competes with a fixed amount of Biotinylated-Substance P. Following incubation, Biotinylated-Substance P and free Substance P in the sample or standard are washed from the plate. Avidin conjugated to Horseradish Peroxidase (Avidin-HRP) is then added to each well and incubated. TMB substrate solution is then added to each well, and after incubation, the enzyme-substrate reaction is terminated by the addition of sulfuric acid stop solution. The color change is measured spectrophotometrically at a wavelength of 450nm, and the concentration of Substance P in the samples is then determined by comparing the O.D. 450nm of the samples to the standard curve. The O.D. 450nm is inversely proportional to the concentration of Substance P in the sample.

Piattaforma

Pre-coated Microplate (12 x 8 well strips)

Tipo di rilevamento

Colourmetric

Instrument

Colorimetric Microplate Reader

Tipo di campione

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Sensibilità

4.26 pg/ml

Campo di rilevamento

15.6-1000 pg/ml

Tempo del kit

1 - 2.5 Hours

Reattività

Human

Recovery

Sample Type	n	Range	Average
Serum	5	80% - 102%	91%
EDTA Plasma	5	81% - 99%	90%
Heparin Plasma	5	80% - 89%	84%

Linearity

Sample Type	1:2	1:4	1:8	1:16
Serum (n=5)	87-91%	87-107%	74-101%	92-97%
EDTA Plasma (n=5)	90-105%	84-101%	90-101%	79-108%
Heparin Plasma (n=5)	84-95%	92-105%	82-105%	89-91%

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Note generali

Information online is representative. Always refer to the Product Manual inside the kit.

Conservazione

Store at +4°C. Do not use past expiration date!

Sinonimi

C-terminal-flanking peptide, Hs.2563, neurokinin 1, neurokinin 2, neurokinin A, neurokinin alpha, Neuromedin L, neuropeptide gamma, neuropeptide K, NK2, NKA, NKNA, NPK, PPT, preprotachykinin, protachykinin, protachykinin-1, Substance K, SubstanceP, TAC1, TAC2, TAC2, formerly, Tachykinin 1, tachykinin 2, tachykinin 2, formerly, Tachykinin precursor 1, tachykinin, precursor 1 (substance K, substance P, neurokinin 1, neurokinin 2, neuromedin L, neurokinin alpha, neuropeptide K, neuropeptide gamma), Tachykinin1, TKN1_HUMAN

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.