Human Resistin ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Resistin in the samples. A monoclonal antibody specific for human Resistin has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for Resistin, and Resistin present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Resistin bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Piattaforma

Microplate

Tipo di rilevamento

Colorimetric

Tipo di campione

Cell culture supernatant, serum and plasma.

Sensibilità

2.67 pg/ml

Campo di rilevamento

31.25 - 2000 pg/ml

Recupero

93% (Average Recovery from Serum Samples).

Specificità

This kit can assay both natural and recombinant human Resistin.

Reattività

Human

Reattività incrociata

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Conservazione

Store at 2 - 8°C.

Sinonimi

Adipose tissue specific secretory factor , Adipose tissue-specific secretory factor, ADSF, C/EBP epsilon regulated myeloid specific secreted cysteine rich protein, C/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 1 , C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, CG5403, Cysteine rich secreted protein A12 alpha like 2 , Cysteine rich secreted protein FIZZ3, Cysteine-rich secreted protein A12-alpha-like 2, Cysteine-rich secreted protein FIZZ3, dri, FIZZ 3, FIZZ3, Found in inflammatory zone 3, HXCP 1, HXCP1, MGC126603, MGC126609, PRO1199, Resistin delta2 , RETN, RETN 1, RETN1, RETN_HUMAN, RSTN, UNQ407 , XCP 1 , XCP1

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.