Human Proprotein Convertase 9 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human PCSK9 in the samples. A monoclonal antibody specific for human PCSK9 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for PCSK9, and PCSK9 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of PCSK9 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Piattaforma

Microplate

Tipo di rilevamento

Colorimetric

Tipo di campione

Cell culture supernatant, serum and plasma.

Sensibilità

3.39 pg/ml

Campo di rilevamento

62.50 - 4000 pg/ml

Specificità

This kit can assay both natural and recombinant human PCSK9.

Reattività

Human

Reattività incrociata

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Conservazione

Store at 2 - 8°C.

Sinonimi

Convertase subtilisin/kexin type 9 preproprotein, FH3, HCHOLA3, Hypercholesterolemia autosomal dominant 3, LDLCQ1, NARC 1, NARC-1, NARC1, Neural apoptosis regulated convertase 1, Neural apoptosis-regulated convertase 1, PC 9, PC9, PCSK 9, PCSK9, PCSK9_HUMAN, Proprotein convertase PC9, Proprotein convertase subtilisin/kexin type 9, PSEC0052, Subtilisin/kexin like protease PC9, Subtilisin/kexin-like protease PC9

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.