Umano NKB Kit Elisa (A2240)

$585
Spese di spedizione
Tempi di consegna
7-10 Giorni lavorativi
Telefono
+1 (314) 370-6046
Lun - Ven, 8:00 - 16:00 AST
E-mail
orders@antibodies.com
Nome
Human NKB ELISA Kit
Descrizione
Human NKB ELISA Kit is a competitive (quantitative) Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of human NKB in serum, plasma or other biological fluids.
Tipo di kit
Competitive (quantitative)
Principio di kit
Human NKB ELISA Kit employs the Competitive Enzyme Immunoassay (ELISA) technique for the quantitative measurement ofhumanNKB in serum, plasma or other biological fluids. A monoclonal antibody specific for NKB has been pre-coated onto a 96-well microtiter plate. During the incubation, NKB in the sample or standard competes with a fixed amount of Biotinylated-NKB. Following incubation, Biotinylated-NKB and free NKB in the sample or standard are washed from the plate. Avidin conjugated to Horseradish Peroxidase (Avidin-HRP) is then added to each well and incubated. TMB substrate solution is then added to each well, and after incubation, the enzyme-substrate reaction is terminated by the addition of sulfuric acid stop solution. The color change is measured spectrophotometrically at a wavelength of 450nm, and the concentration of NKB in the samples is then determined by comparing the O.D. 450nm of the samples to the standard curve. The O.D. 450nm is inversely proportional to the concentration of NKB in the sample.
Piattaforma
Pre-coated Microplate (12 x 8 well strips)
Tipo di rilevamento
Colourmetric
Instrument
Colorimetric Microplate Reader
Tipo di campione
Serum, plasma or other biological fluids.
Sensibilità
3.12 pg/ml
Campo di rilevamento
12.5-800 pg/ml
Tempo del kit
1 - 2.5 Hours
Reattività
Human
Recovery
Sample Type n Range Average
Serum 5 80% - 102% 91%
EDTA Plasma 5 81% - 100% 90%
Heparin Plasma 5 80% - 89% 84%
Linearity
Sample Type 1:2 1:4 1:8 1:16
Serum (n=5) 87-91% 87-107% 74-101% 92-97%
EDTA Plasma (n=5) 90-105% 84-101% 90-101% 79-108%
Heparin Plasma (n=5) 84-95% 92-105% 82-105% 89-91%
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Note generali
Information online is representative. Always refer to the Product Manual inside the kit.
Conservazione
Store at +4°C. Do not use past expiration date!
Sinonimi
Gamma tachykinin 3, Neurokinin B, Neurokinin B like protein, Neurokinin B protein, Neurokinin beta, Neurokinin-B, NeurokininB, Neuromedin K, Neuromedin-K, NeuromedinK, NKNB, Preprotachykinin B, PreprotachykininB, PRO 1155, PRO1155, TAC 3, TAC3, Tachykinin 3, Tachykinin3, TKNK_HUMAN, ZNEUROK 1, ZNEUROK1
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
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