Umano M-CSF Kit Elisa (A1483)

Human M-CSF ELISA Kit

Sandwich (quantitative)

Human M-CSF ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human M-CSF in the samples. A monoclonal antibody specific for human M-CSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and M-CSF present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for M-CSF is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of M-CSF bound in the initial step. The color development is stopped and the intensity of the color is measured.

Microplate

Colorimetric

Cell culture supernatant, serum and plasma.

31.25 - 2000 pg/ml

102% (Average Recovery from Serum Samples).

This kit can assay both natural and recombinant human M-CSF.

Human

This assay has shown no significant cross-reactivity.

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Store at 2 - 8°C.

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.