Umano IL-4 Kit Elisa (A1470)

Human IL-4 ELISA Kit

Sandwich (quantitative)

Human IL-4 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human IL-4 in the samples. A monoclonal antibody specific for human IL-4 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for IL-4, and IL-4 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-4 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

Cell culture supernatant, serum and plasma.

7.81 - 500 pg/ml

94% (Average Recovery from Serum Samples).

This kit can assay both natural and recombinant human IL-4.

Human

This assay has shown no significant cross-reactivity.

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Store at 2 - 8°C.

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.