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Ricombinante Anti-Umano IgG4 Anticorpo [RM120] (Biotin) (A121272)

ELISA - Anti-Human IgG4 Antibody (Biotin) [RM120] (A121272) - Antibodies.com ELISA - Anti-Human IgG4 Antibody (Biotin) [RM120] (A121272) - Antibodies.com Western Blot - Anti-Human IgG4 Antibody (Biotin) [RM120] (A121272) - Antibodies.com ELISA - Anti-Human IgG4 Antibody (Biotin) [RM120] (A121351) - Antibodies.com Immunohistochemistry - Anti-Human IgG4 Antibody (Biotin) [RM120] (A121272) - Antibodies.com
Anticorpo Ricombinante
$480

Anticorpo monoclonale [RM120] di coniglio contro IgG4 (Biotin) per WB, ICC, IHC e Flow Cytometry.

Garanzia al 100%
Garanzia di corrispondenza del prezzo

Spese di spedizione
$40
Tempi di consegna
4-6 Giorni lavorativi

Telefono
+1 (314) 370-6046
Lun - Ven, 8:00 - 16:00 AST
E-mail
orders@antibodies.com

Nome
Anti-Human IgG4 Antibody [RM120] (Biotin)
Descrizione
Recombinant rabbit monoclonal [RM120] antibody to Human IgG4 (Biotin).
Specificità
This antibody recognizes the heavy chain of human IgG4.
Applicazioni
WB, ICC, IHC, Flow Cytometry
Diluizioni consigliate
ELISA: 50-200 ng/well (Capture) and 0.05-0.2 µg/ml (Detection), ICC: 110 µg/ml, IHC: 110 µg/ml
Reattività
Human
Reattività incrociata
There is no cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
Immunogeno
Synthetic peptide corresponding to the hinge region of human IgG4.
Specie ospite
Rabbit
Clonalità
Monoclonal
Clona ID
RM120
Isotipo
IgG
Coniugare

Biotin

Purificazione
Protein A chromatography.
Concentrazione
1 mg/ml
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
Ig gamma 4 chain C region, IGHG4, immunoglobulin heavy chain constant region gamma 4, Immunoglobulin heavy constant gamma 4 (G4m marker), MGC117419
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
ELISA - Anti-Human IgG4 Antibody (Biotin) [RM120] (A121272) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Anti-Human IgG4 Antibody [RM120] (Biotin) (A121272)
Sandwich ELISA using Anti-Human IgG4 Antibody [RM120] as the capture antibody, and Anti-Human Ig Light Chain Antibody [RM129] (Biotin) as the detection antibody, followed by an AP conjugated streptavidin.
ELISA - Anti-Human IgG4 Antibody (Biotin) [RM120] (A121272) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Anti-Human IgG4 Antibody [RM120] (Biotin) (A121272)
Sandwich ELISA using Anti-Human IgG4 Antibody [RM120] as the capture antibody, and Anti-Human Ig Light Chain Antibody [RM129] (Biotin) as the detection antibody, followed by an AP conjugated streptavidin.
Western Blot - Anti-Human IgG4 Antibody (Biotin) [RM120] (A121272) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Western Blot - Anti-Human IgG4 Antibody [RM120] (Biotin) (A121272)
Western blot of human, mouse, rat, and goat IgG shows Anti-Human IgG4 Antibody [RM120] (Biotin) reacts to human IgG4, in both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced) forms. There is no cross reactivity with other isotypes of human IgG, mouse IgG, rat IgG, or goat IgG.
ELISA - Anti-Human IgG4 Antibody (Biotin) [RM120] (A121351) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Anti-Human IgG4 Antibody [RM120] (Biotin) (A121272)
A titer ELISA using Anti-Human IgG4 Antibody [RM120]. The plate was coated with different amounts of human IgG4. A serial dilution of Anti-Human IgG4 Antibody [RM120] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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