Human IGF-1R ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human IGF-1R in the samples. A monoclonal antibody specific for human IGF-1R has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for IGF-1R, and IGF-1R present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IGF-1R bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Piattaforma

Microplate

Tipo di rilevamento

Colorimetric

Tipo di campione

Cell culture supernatant, serum and plasma.

Sensibilità

13.27 pg/ml

Campo di rilevamento

218.75 - 14000 pg/ml

Recupero

91% (Average Recovery from Serum Samples).

Specificità

This kit can assay both natural and recombinant human IGF-1R.

Reattività

Human

Reattività incrociata

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Conservazione

Store at 2 - 8°C.

Sinonimi

CD221, CD221 antigen, IGF 1 receptor, IGF 1R, IGF I receptor, IGF-I receptor, IGF1 Receptor, IGF1R_HUMAN, IGFIR, IGFIRC, IGFR, Insulin like growth factor 1 receptor, Insulin like growth factor 1 receptor precursor, Insulin-like growth factor 1 receptor beta chain, Insulin-like growth factor I receptor, JTK13, MGC142170, MGC142172, MGC18216, Soluble IGF1R variant 1, Soluble IGF1R variant 2

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.