Sandwich ELISA using Anti-Human IgA2 Antibody [RM125] as the capture antibody (100ng/well), and Anti-Human Ig Light Chain Antibody [RM129] (Biotin) as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
Sandwich ELISA using Anti-Human IgA2 Antibody [RM125] as the capture antibody (100ng/well), and Anti-Human Ig Light Chain Antibody [RM129] (Biotin) as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
ELISA of human immunoglobulins shows Anti-Human IgA2 Antibody [RM125] reacts only to Human IgA2. There is no cross reactivity with Human IgA1, IgG, IgM, IgD, or IgE. The plate was coated with 50ng/well of different immunoglobulins. 200ng/ml, 50ng/ml, or 10ng/ml of Anti-Human IgA2 Antibody [RM125] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
A titer ELISA using Anti-Human IgA2 Antibody [RM125]. The plate was coated with different amounts of human IgA2. A serial dilution of Anti-Human IgA2 Antibody [RM125] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.