Umano HMGA2 Kit Elisa (A5544)

Human HMGA2 ELISA Kit

Human HMGA2 ELISA Kit is a sandwich (quantitative) Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human HMGA2 in tissue homogenates, cell lysates or other biological fluids.

Sandwich (quantitative)

Human HMGA2 ELISA Kit employs the Sandwich Enzyme Immunoassay (ELISA) technique for the quantitative measurement of human HMGA2 in human. An antibody specific for HMGA2 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the HMGA2 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-HMGA2 Antibody, which binds the captured HMGA2 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and Avidin conjugated to Horseradish Peroxidase (Avidin-HRP) is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of HMGA2 captured in each well. The concentration of HMGA2 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colourmetric

Colorimetric Microplate Reader

Tissue homogenates, cell lysates or other biological fluids.

0.114 ng/ml

0.312-20 ng/ml

Human

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Information online is representative. Always refer to the Product Manual inside the kit.

Store at +4°C. Do not use past expiration date!

BABL, High mobility group (nonhistone chromosomal) protein isoform I C, High mobility group (nonhistone chromosomal) protein isoform IC, High Mobility Group AT hook 2, High Mobility Group AT hook protein 2, High mobility group AT-hook protein 2, High mobility group protein HMGI C, High mobility group protein HMGI-C, High mobility group protein HMGIC, High mobility group protein I, isoform C, HMGA 2, HMGA2_HUMAN, HMGI C, HMGIC, LIPO, Non histone chromosomal architectural protein HMGI C, Non histone chromosomal architectural protein HMGIC, Pygmy, STQTL9