Human HGF ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human HGF in the samples. A monoclonal antibody specific for human HGF has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for HGF, and HGF present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of HGF bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Piattaforma

Microplate

Tipo di rilevamento

Colorimetric

Tipo di campione

Cell culture supernatant, serum and plasma.

Sensibilità

1.99 pg/ml

Campo di rilevamento

31.25 - 2000 pg/ml

Recupero

95% (Average Recovery from Serum Samples).

Specificità

This kit can assay both natural and recombinant human HGF.

Reattività

Human

Reattività incrociata

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Conservazione

Store at 2 - 8°C.

Sinonimi

DFNB39, F TCF, Fibroblast derived tumor cytotoxic factor, Hepatocyte growth factor, Hepatocyte growth factor (hepapoietin A; scatter factor), Hepatocyte growth factor beta chain, Hepatocyte growth factor precursor, Hepatopoietin A, Hepatopoietin-A, HGFB, HGF_HUMAN, HPTA, Lung fibroblast derived mitogen , OTTHUMP00000161349, OTTHUMP00000206710, OTTHUMP00000206711, OTTHUMP00000206712, OTTHUMP00000206713, OTTHUMP00000206730, Scatter factor, SF

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.