Umano Growth Hormone Kit Elisa (A1441)

Human Growth Hormone ELISA Kit

Sandwich (quantitative)

Human Growth Hormone ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Growth Hormone in the samples. A monoclonal antibody specific for human Growth Hormone has been immobilized onto microwells. Standard or samples are pipetted into the wells, and Growth Hormone present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for Growth Hormone is added to the wells. Following a wash to remove any unbound detect antibody, streptavidin-HRP is added. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Growth Hormone bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

Cell culture supernatant, serum and plasma.

62.50 - 4000 pg/ml

105% (Average Recovery from Serum Samples).

This kit can assay both natural and recombinant human Growth Hormone.

Human

This assay has shown no significant cross-reactivity.

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Store at 2 - 8°C.

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.