Human CCL26 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Eotaxin-3 in the samples. A monoclonal antibody specific for human Eotaxin-3 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for Eotaxin-3, and Eotaxin-3 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Eotaxin-3 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Piattaforma

Microplate

Tipo di rilevamento

Colorimetric

Tipo di campione

Cell culture supernatant, serum and plasma.

Sensibilità

1.44 pg/ml

Campo di rilevamento

15.63 - 1000 pg/ml

Recupero

94% (Average Recovery from Serum Samples).

Specificità

This kit can assay both natural and recombinant human Eotaxin-3.

Reattività

Human

Reattività incrociata

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Conservazione

Store at 2 - 8°C.

Sinonimi

C-C motif chemokine 26, CC chemokine IMAC, CCL 26, CCL26_HUMAN, Chemokine (C C motif) ligand 26, chemokine N1, Eotaxin 3, Eotaxin-3, IMAC, Macrophage inflammatory protein 4-alpha, MIP 4a, MIP 4alpha, MIP-4-alpha, MIP4a, MIP4alpha, SCYA 26, SCYA26, Small inducible cytokine A26, Small inducible cytokine subfamily A (Cys Cys) member 26, Small inducible cytokine subfamily A member 26, Small-inducible cytokine A26, Thymic stroma chemokine 1, Thymic stroma chemokine-1, TSC 1, TSC-1, TSC1

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.