Human Apolipoprotein B ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Apo B in the samples. A monoclonal antibody specific for human Apo B has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for Apo B, and Apo B present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Apo B bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
Piattaforma
Microplate
Tipo di rilevamento
Colorimetric
Tipo di campione
Cell culture supernatant, serum and plasma.
Sensibilità
74. pg/ml
Campo di rilevamento
312.50 - 20000 pg/ml
Specificità
This kit can assay both natural and recombinant human Apo B.
Reattività
Human
Reattività incrociata
This assay has shown no significant cross-reactivity.
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Conservazione
Store at 2 - 8°C.
Sinonimi
Apo B-100, Apo B-48, APOB, ApoB 100 , ApoB 48, APOB_HUMAN, Apolipoprotein B (including Ag(x) antigen), Apolipoprotein B 100, Apolipoprotein B-48, Apolipoprotein B48, FLDB, LDLCQ4
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
