Human Angiopoietin-2 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human ANG-2 in the samples. A monoclonal antibody specific for human ANG-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and ANG-2 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for ANG-2 is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of ANG-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Piattaforma
Microplate
Tipo di rilevamento
Colorimetric
Tipo di campione
Cell culture supernatant, serum and plasma.
Sensibilità
2.61 pg/ml
Campo di rilevamento
15.63 - 1000 pg/ml
Recupero
92% (Average Recovery from Serum Samples).
Specificità
This kit can assay both natural and recombinant human ANG-2.
Reattività
Human
Reattività incrociata
This assay has shown no significant cross-reactivity.
