Umano Alpha-fetoprotein Kit Elisa (A1543)

Human Alpha-fetoprotein ELISA Kit

Sandwich (quantitative)

Human AFP ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human AFP in the samples. A monoclonal antibody specific for human AFP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells for the first incubation. After washing away any unbound substances, a HRP-linked detect antibody specific for AFP is added to the wells. After washing, substrate solution is added to the wells and color develops in proportion to the amount of AFP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Microplate

Colorimetric

Cell culture supernatant, serum and plasma.

15.63 - 1000 pg/ml

98% (Average Recovery from Serum Samples).

This kit can assay both natural and recombinant human AFP.

Human

This assay has shown no significant cross-reactivity.

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Store at 2 - 8°C.

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.