This Adiponectin enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-Adiponectin Antibody. Standards or samples are then added to the microtire plate wells and Adiponectin, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of Adiponectin present in the sample, Anti-Adiponectin Antibody (Biotin) is added to each well to "sandwich" the Adiponectin immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Adiponectin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Piattaforma

Microplate

Tipo di rilevamento

Colorimetric

Tipo di campione

Serum, plasma, body fluids, tissue lysates, and cell culture supernatants.

Sensibilità

< 10 pg/ml

Campo di rilevamento

1.56-100 ng/ml

Reattività

Human

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Conservazione

Store at 2-8°C for 6 months or at -20°C for 12 months.

Sinonimi

30 kDa adipocyte complement related protein, 30 kDa adipocyte complement-related protein, ACDC, ACRP30, Adipocyte, Adipocyte C1q and collagen domain containing protein, Adipocyte complement related 30 kDa protein, Adipocyte complement related protein of 30 kDa, Adipocyte complement-related 30 kDa protein, adipocyte-specific secretory protein, Adiponectin precursor, adiponectin, C1Q and collagen domain containing, Adipoq, Adipose most abundant gene transcript 1, Adipose most abundant gene transcript 1 protein, Adipose specific collagen like factor, ADIPO_HUMAN, ADIPQTL1, ADPN, APM 1, apM-1, APM1, C1q and collagen domain-containing protein, GBP28, Gelatin binding protein , Gelatin binding protein 28, Gelatin-binding protein

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.