Western Blot - Anti-HSP60 Antibody [LK2] (A305090)
Western blot analysis of human Heat Shocked cervical cancer cell line (HeLa) lysate showing detection of Hsp60 protein using Anti-HSP60 Antibody [LK2] (A305090) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.
Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-HSP60 Antibody [LK2] (A305090) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: Cytoplasmic Staining.
Immunohistochemistry analysis of human colon carcinoma, fixed in formalin. The Primary Antibody used was Anti-HSP60 Antibody [LK2] (A305090) at 1:100,000 for 12 hours at 4°C. The secondary antibody used was Biotin Goat Anti-Mouse at 1:2000 for 1 hour at room temperature. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at room temperature. Localization: Inflammatory cells. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..
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