Anti-HSP47 Antibody [1C4-1A6] (A304884)

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-HSP47 Antibody [1C4-1A6] (A304884), at 1:100 for 12 hours at 4°C. The secondary antibody used was APC Goat Anti-Mouse (red) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Endoplasmic reticulum lumen. Cytoplasm. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-Hsp47 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Western blot analysis of human Epithelial cell (A431) lysates showing detection of ~47 kDa Hsp47 protein using Anti-HSP47 Antibody [1C4-1A6] (A304884) at 1:250 1:1,000 for 1 hour at room temperature. Lane 1: MW ladder. Lane 2: Anti-Hsp47 (1:250). Lane 3: Anti-Hsp47 (1:500). Lane 4: Anti-Hsp47 (1:1000). Load: 20 µg. Block: 5% milk + TBST for 1 hour at room temperature. The secondary antibody used was HRP Goat Anti-Mouse at 1:50 for 1 hour at room temperature. Color Development: TMB solution for 10 min at room temperature. Predicted/Observed Size: ~47 kDa.

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-HSP47 Antibody [1C4-1A6] (A304884), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Endoplasmic reticulum lumen. Cytoplasm. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-Hsp47 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.