Anti-HSF1 Anticorpo [4B4] (A305103)

Name: Anti-HSF1 Antibody [4B4]
Brand: Antibodies.com
SKU: A305103
Price: 500.0 USD
Availability: InStock

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$500

Anticorpo monoclonale [4B4] di ratto contro HSF1 per WB, IHC, ICC/IF, IP, ELISA e Gel Shift.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

Garanzia di qualità al 100%

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Descrizione Prodotto

Nome

Anti-HSF1 Antibody [4B4]

Descrizione

Rat monoclonal [4B4] antibody to HSF1.

Specificità

Bands correspond to unstressed cell lysates and heat shocked cell lysates.

Applicazioni

WB, IHC, ICC/IF, IP, ELISA, Gel Shift

Diluizioni consigliate

WB: 1:1,000, IHC: 1:2,000, ICC/IF: 1:100

Reattività

Human, Mouse, Rat, Bovine, Guinea Pig, Hamster, Monkey, Rabbit

Immunogeno

Purified recombinant mouse HSF1 protein, epitope mapping to amino acids 425-439.

Specie ospite

Rat

Clonalità

Monoclonal

Clona ID

4B4

Isotipo

IgG1

Coniugare

Unconjugated

Purificazione

Protein G purification.

Concentrazione

1 mg/ml

Peso molecolare

~85 kDa / ~95 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.1% Sodium Azide

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

Heat shock factor 1, Heat shock factor protein 1, Heat shock transcription factor 1, HSF 1, HSF1_HUMAN, HSTF 1, HSTF1

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (4)

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Figure 1: Western Blot - Anti-HSF1 Antibody [4B4] (A305103)

Western blot analysis of human Breast adenocarcinoma cell line (MCF7) showing detection of ~65 kDa HSF1 protein using Anti-HSF1 Antibody [4B4] (A305103) at 1:1,000 for 2 hours at room temperature. Lane 1: MW ladder. Lane 2: HSF1 null lysate prepared from mouse embryonic fibroblasts. Lane 3: MCF7 lysate (5 µg). Lane 4: MCF7 lysate (10 µg). Lane 5: MCF7 lysate (20 µg). Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Goat Anti-Rat IgG: HRP for 1 hour at room temperature. Predicted/Observed Size: ~65 kDa.

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Figure 2: Immunohistochemistry - Anti-HSF1 Antibody [4B4] (A305103)

Immunohistochemistry analysis of human breast carcinoma, fixed in 10% formalin solution for 20 hours at room temperature. The Primary Antibody used was Anti-HSF1 Antibody [4B4] (A305103) at 1:2000 for 40 min. The secondary antibody used was Dako labeled Polymer HRP Anti-rat IgG, DAB Chromogen (brown) (Dako Envision+ System) for 30 minutes at room temperature. Counterstain: Mayer's Hematoxylin (purple/blue) nuclear stain for 1 minute at room temperature. Localization: Nuclear. Magnification: 100X.

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Figure 3: Immunofluorescence - Anti-HSF1 Antibody [4B4] (A305103)

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-HSF1 Antibody [4B4] (A305103), at 1:100 for 12 hours at 4°C. The secondary antibody used was APC Goat Anti-Rat (red) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cytoplasm. Localizes to the nucleus upon activation. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-HSF1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

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Figure 4: Immunofluorescence - Anti-HSF1 Antibody [4B4] (A305103)

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-HSF1 Antibody [4B4] (A305103), at 1:100 for 12 hours at 4°C. The secondary antibody used was R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cytoplasm. Localizes to the nucleus upon activation. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-HSF1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.