Horse POMC ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of horse POMC in serum, plasma, saliva or other biological fluids.

Tipo di kit

Sandwich (quantitative)

Principio di kit

Horse POMC ELISA Kit (A326186) employs the sandwich enzyme immunoassay technique for the quantitative measurement of horse POMC in serum, plasma, saliva or other biological fluids. An antibody specific for POMC has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the POMC present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-POMC Antibody, which binds the captured POMC present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of POMC captured in each well. The concentration of POMC can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Piattaforma

Pre-coated Microplate (12 x 8 well strips)

Tipo di rilevamento

Colourmetric

Instrument

Colorimetric Microplate Reader

Tipo di campione

Serum, plasma, saliva or other biological fluids.

Sensibilità

45 pg/ml

Campo di rilevamento

125-8000 pg/ml

Tempo del kit

4h 30m

Reattività

Horse

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Note generali

Information online is representative. Always refer to the Product Manual inside the kit.

Conservazione

Store kit components at +4°C or -20°C as instructed. Do not use past expiration date!

Sinonimi

ACTH, Adrenocorticotropic hormone, Adrenocorticotropin, alpha melanocyte stimulating hormone, Alpha MSH, Alpha-MSH, beta endorphin, Beta LPH, beta melanocyte stimulating hormone, Beta MSH, Beta-LPH, Beta-MSH, CLIP, COLI_HUMAN, Corticotropin like intermediary peptide, Corticotropin lipotropin, Corticotropin-lipotropin, Gamma MSH, Gamma-LPH, Gamma-MSH, Lipotropin beta, Lipotropin gamma, LPH, Melanotropin, Melanotropin alpha, Melanotropin beta, Melanotropin gamma, met enkephalin, Met-enkephalin, MSH, NPP, POC, pro ACTH endorphin, Proopiomelanocortin, Proopiomelanocortin preproprotein

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.