Anti-HMGB2 Anticorpo [ARC1460] (A307358)

Name: Anti-HMGB2 Antibody [ARC1460]
Brand: Antibodies.com
SKU: A307358
Price: 470.0 USD
Availability: InStock

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$470

Anticorpo monoclonale [ARC1460] di coniglio contro HMGB2 per WB, IHC, ICC/IF, IP e ChIP.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-HMGB2 Antibody [ARC1460]

Descrizione

Rabbit monoclonal [ARC1460] antibody to HMGB2.

Applicazioni

WB, IHC, ICC/IF, IP, ChIP

Diluizioni consigliate

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000, ChIP: 1:500-1:1,000, CUT&Tag: 1⁶ cells/1µg

Reattività

Human, Mouse, Rat

Immunogeno

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HMGB2 (P26583).

Sequenza

MGKGDPNKPRGKMSSYAFFVQTCREEHKKKHPDSSVNFAEFSKKCSERWKTMSAKEKSKFEDMAKSDKARYDREMKNYVPPKGDKKGKKKDPNAPKRPPS

Specie ospite

Rabbit

Clonalità

Monoclonal

Clona ID

ARC1460

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

24 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

C80539, High mobility group (nonhistone chromosomal) protein 2, High mobility group box 2, High mobility group protein 2, High mobility group protein B2, HMG 2, HMG B2, HMG-2, HMG2, HMGB2_HUMAN

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (9)

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Figure 1: Anti-HMGB2 Antibody [ARC1460] (A307358)

CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina from 10⁵ K562 cells with 1µg of Anti-HMGB2 Antibody [ARC1460] (A307358), and Goat Anti-Rabbit IgG H&L Antibody. The CUT&Tag results indicate the enrichment pattern of HMGB2 in representative gene loci (PTBP1), as shown in figure.

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Figure 2: Western Blot - Anti-HMGB2 Antibody [ARC1460] (A307358)

Western blot analysis of extracts of various cell lines, using Anti-HMGB2 Antibody [ARC1460] (A307358) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 3: Immunohistochemistry - Anti-HMGB2 Antibody [ARC1460] (A307358)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-HMGB2 Antibody [ARC1460] (A307358) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-HMGB2 Antibody [ARC1460] (A307358)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-HMGB2 Antibody [ARC1460] (A307358) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.