Dilutions
Flow Cytometry: 1-4 µg/ml; Intracellular staining, IHC-Fr: 10 µg/ml; Positive Control: placenta, IHC-P: 10 µg/ml; Positive Control: placenta, ELISA: Positive Control: HeLa/HLA-G5 transfectants cell lysate, HeLa/HLA-G5 cell supernatant; negative control: HeLa cell lysate. This antibody has been tested as the capture antibody in a sandwich ELISA for analysis of soluble HLA-G in combination with antibody W6/32 (cat. no. 1B-422-C100), WB: Positive Control: JEG-3 cell lysate; Reducing conditions, 12% AA SDS-PAGE.