This antibody recognizes a common extracellular epitope on human HLA DR which is dependent on the association of alpha and beta chains. DR is the isotype of human MHC Class II molecules expressed on antigen-presenting cells (APC, dendritic cells, B lymphocytes, monocytes, and macrophages).

Applicazioni

Flow Cytometry

Diluizioni consigliate

Flow Cytometry: The reagent is designed for analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Reattività

Human

Immunogeno

Thymocyte membrane.

Specie ospite

Mouse

Clonalità

Monoclonal

Clona ID

MEM-12

Isotipo

IgG1

Coniugare

Excitation: 565nm, Emission: 578nm

Purificazione

This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Conservazione

Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!

Sinonimi

HLA DP, HLA DR + HLA DP, MHC Class II

Controlli isotipo

Mouse IgG1 [MOPC-21] (PE) (A86199)

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (1)

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Figure 1: Flow Cytometry - Anti-HLA DR Antibody [MEM-12] (PE) (A85811)

Surface staining of human peripheral blood cells with Anti-HLA DR Antibody (A85811).

Pubblicazioni per questo clone (1)

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Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens.

Riferimenti: Anti-HLA DR Antibody [MEM-12] (PE) (ab28327)

Astratto:

Seven murine monoclonal antibodies were prepared that react with human class II antigens. Four of them (HL-39, MEM-12, MEM-24G, and MEM-32B: react with a monomorphic determinant dependent on association of heavy and light chains of DR antigens, two others (HL-38 and HL-40) recognize a monomorphic determinant localized on the light chains of DR and DP antigens. The antibody HL-37 is directed against a determinant present on DQ1 and DQ3, but not DQ2 molecules; at least in the case of DQ1, the epitope recognized is located on the light chain.

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