Anti-Histone H3.3 (phospho Thr3) Anticorpo [ARC1662] (A307002)

Name: Anti-Histone H3.3 (phospho Thr3) Antibody [ARC1662]
Brand: Antibodies.com
SKU: A307002
Price: 540.0 USD
Availability: InStock

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$540

Anticorpo monoclonale [ARC1662] di coniglio contro Histone H3.3 (phospho Thr3) per WB.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-Histone H3.3 (phospho Thr3) Antibody [ARC1662]

Descrizione

Rabbit monoclonal [ARC1662] antibody to Histone H3.3 (phospho Thr3).

Applicazioni

Diluizioni consigliate

WB: 1:500-1:2,000

Reattività

Human, Mouse, Rat

Immunogeno

A synthetic phosphorylated peptide around T3 of human Histone H3 (P84243).

Sequenza

MARTK

Specie ospite

Rabbit

Clonalità

Monoclonal

Clona ID

ARC1662

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

16 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

H3 histone family 3A, H3 histone family 3B, H3 histone, family 3B (H3.3B), H3.3, H3.3A, H3.3B, H33_HUMAN, H3F3, H3F3A, H3f3b, Histone H3.3Q, Histone H3.A, Histone H3.B, MGC87782, MGC87783

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (3)

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Figure 1: Western Blot - Anti-Histone H3.3 (phospho Thr3) Antibody [ARC1662] (A307002)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-Histone H3.3 (phospho Thr3) Antibody [ARC1662] (A307002) at 1:1,000 dilution. NIH/3T3 cells were treated by Nocodazole (50 ng/ml) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.

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Figure 2: Western Blot - Anti-Histone H3.3 (phospho Thr3) Antibody [ARC1662] (A307002)

Western blot analysis of extracts of HeLa cells, using Anti-Histone H3.3 (phospho Thr3) Antibody [ARC1662] (A307002) at 1:1,000 dilution. HeLa cells were treated by nocodazole (50 ng/ml) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 3: Western Blot - Anti-Histone H3.3 (phospho Thr3) Antibody [ARC1662] (A307002)

Western blot analysis of extracts of C6 cells, using Anti-Histone H3.3 (phospho Thr3) Antibody [ARC1662] (A307002) at 1:1,000 dilution. C6 cells were treated by Nocodazole (50 ng/ml) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.