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Histone H2B Cell Based Kit Elisa (A103372)

Nome
Histone H2B Cell Based ELISA Kit
Tipo di kit
Cell-based (qualitative)
Principio di kit
This Histone H2B Cell Based ELISA Kit allows for the detection of Histone H2B and the effects that certain stimulation conditions have on Histone H2B expression in different cell lines. Qualitative determination of Histone H2B concentration is achieved by an indirect ELISA format. In essence, the Histone H2B is captured by Anti-Histone H2B Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this Histone H2B Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Piattaforma
Microplate
Tipo di rilevamento
Colorimetric
Tipo di campione
Adherent cells and suspension cells.
Campo di rilevamento
> 5000 Cells
Tempo del kit
4 hours 30 minutes
Reattività
Human, Mouse
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Conservazione
Store at + 4°C. Stable for 6 months.
Sinonimi
GL105, H2B, H2B 1A, H2B GL105, H2B histone family, H2B histone family member O, H2B histone family member Q, H2B histone family member S, H2B type 12, H2B-GL105, H2B.1, H2B.1 B, H2b.1A, H2B.b, H2B.c, H2B.d, H2B.e, H2B.f, H2B.j, H2B.q, H2B/a, H2B/b, H2B/c, H2B/d, H2B/e, H2B/f, H2B/g, H2B/h, H2B/j, H2B/l, H2B/o, H2B/q, H2B1_YEAST, H2B2E_HUMAN, H2B2f, H2B2F_HUMAN, H2Ba, H2Bf, H2BFB, H2BFC, H2BFD, H2BFE, H2BFF, H2BFJ, H2BFO, H2BFQ, H2BFS, H2BGL105, H2BQ, HIRIP2, HIST1H2BB, HIST1H2BD, HIST1H2BH, HIST1H2BL, HIST1H2BM, HIST1H2BN, HIST2H2BE, HIST2H2BF, Histone 2 H2be, histone cluster 2, H2be, histone H2B GL105, histone H2B type 1, Histone H2B type 1 B, Histone H2B type 1 D, Histone H2B type 1 H, Histone H2B type 1 L, Histone H2B type 1 M, Histone H2B type 1 N, Histone H2B type 1-B, Histone H2B type 1-C/E/F/G/I, Histone H2B type 1-D, Histone H2B type 1-H, Histone H2B type 1-L, Histone H2B type 1-M, Histone H2B type 1-N, Histone H2B type 2 E, Histone H2B type 2-E, Histone H2B type 2-F, histone H2B type 2E, Histone H2B-GL105, Histone H2B.1, Histone H2B.1 A, Histone H2B.2, Histone H2B.a, Histone H2B.b, Histone H2B.f, Histone H2B.g, Histone H2B.h, Histone H2B.k, Histone H2B.l, Histone H2B.n, Histone H2B.q, histone H2BGL105, histone protein, HTB 1, HTB 2, HTB1, Htb1p, HTB2, Htb2p, MGC131639, SPT 12, SPT12, Suppressor of Ty protein 12
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Histone H2B Cell Based ELISA Kit (CB5334) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Histone H2B Cell Based ELISA Kit (CB5334)
Western blot analysis of the lysates from HUVECcells using Anti-Histone H2B antibody.
Protocol Diagram - Histone H2B Cell Based ELISA Kit (CB5334) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Histone H2B Cell Based ELISA Kit (CB5334)
A diagram showing the protocol for Histone H2B Cell Based ELISA Kit.
Protocol Summary - Histone H2B Cell Based ELISA Kit (CB5334) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Histone H2B Cell Based ELISA Kit (CB5334)
A summary of the protocol for Histone H2B Cell Based ELISA Kit.
Western Blot - Control Antibody - Histone H2B Cell Based ELISA Kit (CB5334) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Western Blot - Histone H2B Cell Based ELISA Kit (CB5334)
The mouse monoclonal antibody to GAPDH is used as a positive control inHistone H2B Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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