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Anti-Histone H2B Anticorpo (A309)

1 Pubblicazioni
Identification of histone H2B in the crude extract of fission yeast S. pombe with this anti body.
$480

Anticorpo policlonale di coniglio contro Histone H2B per WB, IP e ChIP.

Garanzia al 100%
Garanzia di corrispondenza del prezzo

Spese di spedizione
$40
Tempi di consegna
4-6 Giorni lavorativi

Telefono
+1 (314) 370-6046
Lun - Ven, 8:00 - 16:00 AST
E-mail
orders@antibodies.com

Nome
Anti-Histone H2B Antibody
Descrizione
Rabbit polyclonal antibody to Histone H2B.
Applicazioni
WB, IP, ChIP
Diluizioni consigliate
WB: 1:1,000
Reattività
Schizosaccharomyces pombe
Immunogeno
Synthetic peptide corresponding to the N-terminal of Schizosaccharomyces pombe Histone H2B protein.
Sequenza
SAAEKKPASKAPAGKA
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare

Unconjugated

Forma del prodotto
Liquid
Formulazione
Supplied as antiserum with 0.05% Sodium Azide.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
GL105, H2B, H2B 1A, H2B GL105, H2B histone family, H2B histone family member O, H2B histone family member Q, H2B histone family member S, H2B type 12, H2B-GL105, H2B.1, H2B.1 B, H2b.1A, H2B.b, H2B.c, H2B.d, H2B.e, H2B.f, H2B.j, H2B.q, H2B/a, H2B/b, H2B/c, H2B/d, H2B/e, H2B/f, H2B/g, H2B/h, H2B/j, H2B/l, H2B/o, H2B/q, H2B1_YEAST, H2B2E_HUMAN, H2B2f, H2B2F_HUMAN, H2Ba, H2Bf, H2BFB, H2BFC, H2BFD, H2BFE, H2BFF, H2BFJ, H2BFO, H2BFQ, H2BFS, H2BGL105, H2BQ, HIRIP2, HIST1H2BB, HIST1H2BD, HIST1H2BH, HIST1H2BL, HIST1H2BM, HIST1H2BN, HIST2H2BE, HIST2H2BF, Histone 2 H2be, histone cluster 2, H2be, histone H2B GL105, histone H2B type 1, Histone H2B type 1 B, Histone H2B type 1 D, Histone H2B type 1 H, Histone H2B type 1 L, Histone H2B type 1 M, Histone H2B type 1 N, Histone H2B type 1-B, Histone H2B type 1-C/E/F/G/I, Histone H2B type 1-D, Histone H2B type 1-H, Histone H2B type 1-L, Histone H2B type 1-M, Histone H2B type 1-N, Histone H2B type 2 E, Histone H2B type 2-E, Histone H2B type 2-F, histone H2B type 2E, Histone H2B-GL105, Histone H2B.1, Histone H2B.1 A, Histone H2B.2, Histone H2B.a, Histone H2B.b, Histone H2B.f, Histone H2B.g, Histone H2B.h, Histone H2B.k, Histone H2B.l, Histone H2B.n, Histone H2B.q, histone H2BGL105, histone protein, HTB 1, HTB 2, HTB1, Htb1p, HTB2, Htb2p, MGC131639, SPT 12, SPT12, Suppressor of Ty protein 12
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Identification of histone H2B in the crude extract of fission yeast S. pombe with this anti body. Clicca sull'immagine per la visualizzazione estesa
Figure 1: Anti-Histone H2B Antibody (63-125)
Anti-Histone H2B Antibody
Histone H2B mutations in inner region affect ubiquitination, centromere function, silencing and chromosome segregation.
Riferimenti: Anti-Histone H2B Antibody (A309)
Astratto:

The reiterated nature of histone genes has hampered genetic approach to dissect the role of histones in chromatin dynamics. We here report isolation of three temperature-sensitive (ts) Schizosaccharomyces pombe strains, containing amino-acid substitutions in the sole histone H2B gene (htb1+). The mutation sites reside in the highly conserved, non-helical residues of H2B, which are implicated in DNA-protein or protein-protein interactions in the nucleosome. In the allele of htb1-72, the substitution (G52D) occurs at the DNA binding loop L1, causing disruption of the gene silencing in heterochromatic regions and lagging chromosomes in anaphase. In another allele htb1-223 (P102L) locating in the junction between alpha3 and alphaC, the mutant residue is in contact with H2A and other histones, leading to structural aberrations in the central centromere chromatin and unequal chromosome segregation in anaphase. The third allele htb1-442 (E34K) near alpha1 displayed little defect. Evidence is provided that monoubiquitinated H2B is greatly unstable in P102L mutant, possibly owing to proteasome-independent destruction or enhanced deubiquitination. Histone H2B thus plays an important role in centromere/kinetochore formation.

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