Anti-Histone H2A.Z Anticorpo [ARC1048] (A306893)

Name: Anti-Histone H2A.Z Antibody [ARC1048]
Brand: Antibodies.com
SKU: A306893
Price: 470.0 USD
Availability: InStock

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$470

Anticorpo monoclonale [ARC1048] di coniglio contro Histone H2A.Z per WB, IP e ChIP.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Tag dei prodotti:

Anticorpo Knockout Convalidato Nuclear Marker

Descrizione Prodotto

Nome

Anti-Histone H2A.Z Antibody [ARC1048]

Descrizione

Rabbit monoclonal [ARC1048] antibody to Histone H2A.Z.

Applicazioni

WB, IP, ChIP

Diluizioni consigliate

WB: 1:500-1:1,000, ChIP: 1:50-1:200

Reattività

Human, Mouse, Rat

Immunogeno

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone H2A.Z (P0C0S5).

Sequenza

MAGGKAGKDSGKAKTKAVSRSQRAGLQFPVGRIHRHLKSRTTSHGRVGATAAVYSAAILEYLTAEVLELAGNASKDLKVKRITPRHLQLAIRGDEELDSL

Specie ospite

Rabbit

Clonalità

Monoclonal

Clona ID

ARC1048

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

15 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

H2A histone family member Z, H2A.z, H2A/z, H2afz, H2AZ, H2AZ_HUMAN, MGC117173

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (3)

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Figure 1: Western Blot - Anti-Histone H2A.Z Antibody [ARC1048] (A306893)

Western blot analysis of extracts of various cell lines, using Anti-Histone H2A.Z Antibody [ARC1048] (A306893) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-Histone H2A.Z Antibody [ARC1048] (A306893)

Western blot analysis of extracts from wild type(WT) and Histone H2A. Z knockout (KO) 293T(KO) cells, using Anti-Histone H2A.Z Antibody [ARC1048] (A306893) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 3: Chromatin Immunoprecipitation - Anti-Histone H2A.Z Antibody [ARC1048] (A306893)

Chromatin immunoprecipitation (ChIP) analysis of extracts of HeLa cells, using Anti-Histone H2A.Z Antibody [ARC1048] (A306893) and Rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.