Histone H1 Cell Based Kit Elisa (A103376)

Name: Histone H1 Cell Based ELISA Kit
Brand: Antibodies.com
SKU: A103376
Price: 550.0 USD
Availability: InStock

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$550

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Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Nome

Histone H1 Cell Based ELISA Kit

Tipo di kit

Cell-based (qualitative)

Principio di kit

This Histone H1 Cell Based ELISA Kit allows for the detection of Histone H1 and the effects that certain stimulation conditions have on Histone H1 expression in different cell lines. Qualitative determination of Histone H1 concentration is achieved by an indirect ELISA format. In essence, the Histone H1 is captured by Anti-Histone H1 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this Histone H1 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.

Piattaforma

Microplate

Tipo di rilevamento

Colorimetric

Tipo di campione

Adherent cells and suspension cells.

Campo di rilevamento

> 5000 Cells

Tempo del kit

4 hours 30 minutes

Reattività

Human, Mouse

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Conservazione

Store at + 4°C. Stable for 6 months.

Sinonimi

dJ221C16.2, dJ221C16.5, DmeH1, H1, H1 0 histone, H1 histone family, H1 histone family member 0, H1 histone family member 1, H1 histone family member 3, H1 histone family member 5, H1 histone family member N testis specific, H1 histone family member O oocyte specific, H1 histone family member X, H1(0), H1.1, H1.2, H1.3, H1.4, H1.5, H10, H14_HUMAN, H1A, H1f0, H1F0 protein, H1F1, H1F2, H1F3, H1F4, H1F5, H1FNT, H1FOO, H1FOO protein, H1FOO_HUMAN, H1FT, H1FV, H1FX, H1oo, H1t, H1T2, H1X, HANP1, His1, HisC, HIST1, HIST1H1A, HIST1H1B, HIST1H1C, HIST1H1D, HIST1H1E, HIST1H1T, Histone, Histone 1 H1a, Histone 1 H1b, Histone 1 H1c, Histone 1 H1d, Histone 1 H1e, Histone 1 H1t, Histone H1', Histone H1.0, Histone H1.1, Histone H1.2, Histone H1.3, Histone H1.4, Histone H1.5, Histone H1a, Histone H1B, Histone H1oo, Histone H1x, MGC116819, MGC126630, MGC126632, MGC126642, MGC138176, MGC138345, MGC15959, MGC3992, MGC50807, MGC5241, MGC8350, Oocyte specific histone H1, Oocyte specific linker histone H1, Oocyte-specific histone H1, Oocyte-specific linker histone H1, osH1, Testicular H1 histone

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (4)

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Figure 1: Western Blot - Histone H1 Cell Based ELISA Kit (CB5332)

Western blot analysis of extracts from COLO cells, using Anti-Histone H1 (Ab-17) Antibody.

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Figure 2: Histone H1 Cell Based ELISA Kit (CB5332)

A diagram showing the protocol for Histone H1 Cell Based ELISA Kit.

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Figure 3: Histone H1 Cell Based ELISA Kit (CB5332)

A summary of the protocol for Histone H1 Cell Based ELISA Kit.

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Figure 4: Western Blot - Histone H1 Cell Based ELISA Kit (CB5332)

The mouse monoclonal antibody to GAPDH is used as a positive control inHistone H1 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.